Anticancer Activity of Bangle Hantu (Zingiber ottensii Val.) Rhizomes on Breast Cancer Cell Lines MCF-7

Ernawati Sinaga1,2,*, Suprihatin2,3, Ida Wiryanti1,2

 1Faculty of Biology, Universitas Nasional, Jakarta, Indonesia; 2Center for Research and Development of Medicinal Plants, Universitas Nasional, Jakarta, Indonesia; 3Faculty of Health Sciences, Universitas Nasional, Jakarta, Indonesia.

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Bangle Hantu (Zingiber ottensii Val.) is one of underutilized Zingiberaceae plants which are abundantly grow in Indonesia. It is not commonly used in Indonesian traditional medicine nor as spice in Indonesian culinary system. To increase its utilization, in this study we investigated anticancer activity of the methanolic extract of the rhizomes on human breast cancer cell line  MCF-7. The antiproliferative effect was measured by comparing the rate of proliferation (doubling time) of cells treated with extract to untreated or control cells using in vitro tetrazolium salt (MTT) assay, and the ability to induce apoptosis was observed using acridine orange-ethidium bromide staining. The results showed that the methanolic extract significantly inhibit the proliferation of MCF-7, showed by no proliferation occured in cells treated with 20 µg/mL extract (the lowest concentration used in the experiment) while only 25% cells left alive after 24 hours incubation with 80 µg/mL extract (the highest concentration used in the experiment). The extract also showed ability to induced apoptosis of MCF-7 cells. From the results we concluded that the methanolic extract of the rhizome of Zingiber ottensii Val. have anticancer activity and could be developed further as source of novel natural anticancer agent.
Anticancer, Zingiber ottensii, MCF-7, antiproliferative, apoptosis

Presented at 4th International Conference on Green Technology, November 2013, Malang, Jawa Timur